A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances
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منابع مشابه
A method to reduce interference by sucrose in the detection of thiobarbituric acid-reactive substances.
A thiobarbituric acid (TBA) reaction for measuring lipid peroxidation products was evaluated for interference by several ingredients commonly used in solutions to prepare or analyze tissue homogenates or subcellular organelles. These included sucrose (up to 100 mM final concentration in the assay medium), Tris-maleate (up to 40 mM), imidazole (up to 20 mM), inorganic phosphate (up to 10 mM), an...
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Malondialdehyde (MDA), used as an oxidative stress marker, is commonly assayed by measuring the thiobarbituric acid reactive substances (TBARS) using HPLC, as an indicator of the MDA concentration. Since the HPLC method, though highly specific, is time-consuming and expensive, usually it is not suitable for the rapid test in large-scale environmental epidemiologic surveys. The purpose of this s...
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ژورنال
عنوان ژورنال: Analytical Biochemistry
سال: 1984
ISSN: 0003-2697
DOI: 10.1016/0003-2697(84)90084-8